0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Application Dilute:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Notes:
Application Notes: Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher
IHC staining of FFPE human lymph node with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant TOP2A antibody (clone rTOP2A/6569). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant TOP2A antibody (clone rTOP2A/6569) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa and 2) Jurkat cell lysate using recombinant TOP2A antibody (clone rTOP2A/6569). Predicted molecular weight ~174 kDa.
SDS-PAGE analysis of purified, BSA-free recombinant TTOP2A antibody (clone rTOP2A/6569) as confirmation of integrity and purity.
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