Application Notes: Optimal dilution of the GTF2H2C antibody should be determined by the researcher
IHC staining of FFPE human breast carcinoma tissue with GTF2H2C antibody (clone PCRP-GTF2H2C-2C9). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast carcinoma tissue with GTF2H2C antibody (clone PCRP-GTF2H2C-2C9). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human HeLa cells using GTF2H2C antibody (green, clone PCRP-GTF2H2C-2C9) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with GTF2H2C antibody (blue, clone PCRP-GTF2H2C-2C9), and unstained cells (red).
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using GTF2H2C antibody (clone PCRP-GTF2H2C-2C9). These results demonstrate the foremost specificity of the PCRP-GTF2H2C-2C9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free GTF2H2C antibody (clone PCRP-GTF2H2C-2C9) as confirmation of integrity and purity.
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