0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Application Dilute:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Notes:
Application Notes: Optimal dilution of the recombinant CD38 antibody should be determined by the researcher
IHC staining of FFPE human prostate adenocarcinoma tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30 min RT. Strong cytoplasmic and membranous staining is observed. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative control: IHC staining of FFPE human brain tissue with recombinant CD38 antibody (clone CD38/6448R) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human Raji cell lysate using recombinant CD38 antibody (clone CD38/6448R). Expected molecular weight: 34-46 kDa depending on glycosylation level.
SDS-PAGE analysis of purified, BSA-free recombinant CD38 antibody (clone CD38/6448R) as confirmation of integrity and purity.
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