0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Application Dilute:
ELISA (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
Application Notes:
Application Notes: Optimal dilution of the MED21 antibody should be determined by the researcher
Immunofluorescent staining of PFA-fixed human U-87 cells using MED21 antibody (green, clone PCRP-MED21-4B5) and phalloidin (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using MED21 antibody (green, clone PCRP-MED21-4B5) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with MED21 antibody (blue, clone PCRP-MED21-4B5), and unstained cells (red).
FACS staining of PFA-fixed human U-87 cells with MED21 antibody (blue, clone PCRP-MED21-4B5), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free MED21 antibody (clone PCRP-MED21-4B5) as confirmation of integrity and purity.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MED21 antibody (clone PCRP-MED21-4B5). These results demonstrate the foremost specificity of the PCRP-MED21-4B5 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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