0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Application Dilute:
ELISA (For coating, order BSA-free format),Western blot: 1-2ug/ml,Immunofluorescence: 0.5-2ug/ml,Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate),Flow cytometry: 0.5-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml
Application Notes:
Application Notes: Optimal dilution of the SP100 antibody should be determined by the researcher
FACS staining of PFA-fixed human HeLa cells with SP100 antibody (blue, clone PCRP-SP100-1B9), and unstained cells (red).
SDS-PAGE analysis of purified, BSA-free SP100 antibody (PCRP-SP100-1B9) as confirmation of integrity and purity.
Immunofluorescent staining of PFA-fixed human HeLa cells using SP100 antibody (green, clone PCRP-SP100-1B9) and phalloidin (red).
IHC staining of FFPE human colon tissue with SP100 antibody (clone PCRP-SP100-1B9) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with SP100 antibody (clone PCRP-SP100-1B9) at 2 ug/ml in PBS for 30 min RT. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using SP100 antibody (clone PCRP-SP100-1B9). These results demonstrate the foremost specificity of the PCRP-SP100-1B9 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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