0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Application Dilute:
Western blot: 2-4ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells
Application Notes:
Application Notes: Optimal dilution of the IRF3 antibody should be determined by the researcher
Western blot testing of human HeLa cell lysate using IRF3 antibody (clone PCRP-IRF3-3B2). Predicted molecular weight ~47 kDa.
SDS-PAGE analysis of purified, BSA-free IRF3 antibody (PCRP-IRF3-3B2) as confirmation of integrity and purity.
FACS staining of PFA-fixed human HeLa cells with IRF3 antibody (blue, clone PCRP-IRF3-3B2) and isotype control (red).
Immunofluorescent staining of PFA-fixed human HeLa cells using IRF3 antibody (green, clone PCRP-IRF3-3B2) and phalloidin (red).
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using IRF3 antibody (clone PCRP-IRF3-3B2). These results demonstrate the foremost specificity of the PCRP-IRF3-3B2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
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