CD3E Recombinant Rabbit Monoclonal Antibody, Clone: [E9C3], Unconjugated

Catalog Number: BYT-ORB1151965
Article Name: CD3E Recombinant Rabbit Monoclonal Antibody, Clone: [E9C3], Unconjugated
Biozol Catalog Number: BYT-ORB1151965
Supplier Catalog Number: orb1151965
Alternative Catalog Number: BYT-ORB1151965-25,BYT-ORB1151965-50,BYT-ORB1151965-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CD3E
Conjugation: Unconjugated
Alternative Names: CD3epsilon, IMD18, T3E, TCRE, CD3, CD3E_HUMAN, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, CD3E_MOUSE,
CD3E Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [E9C3]
Molecular Weight: 20 kDa
UniProt: P07766
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CD3E
Application Dilute: WB=1:1000-5000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000
25 ug total protein per lane of various lysates probed with CD3E monoclonal antibody, unconjugated (orb1151965) at 1:1000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD3E Monoclonal Antibody, Unconjugated (orb1151965) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD3E Monoclonal Antibody, Unconjugated (orb1151965) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD3E Monoclonal Antibody, Unconjugated (orb1151965) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thymus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD3E Monoclonal Antibody, Unconjugated (orb1151965) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with CD3E Monoclonal Antibody, Unconjugated (orb1151965) at 1:300 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.



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