EZH2 Recombinant Rabbit Monoclonal Antibody, Clone: [10A2], Unconjugated
Catalog Number:
BYT-ORB1151968
- Images (7)
| Article Name: | EZH2 Recombinant Rabbit Monoclonal Antibody, Clone: [10A2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1151968 |
| Supplier Catalog Number: | orb1151968 |
| Alternative Catalog Number: | BYT-ORB1151968-25,BYT-ORB1151968-50,BYT-ORB1151968-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human EZH2 |
| Conjugation: | Unconjugated |
| Alternative Names: | ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2, Enx1h, mKIAA4065, EZH2_HUMAN, EZH2, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, 2.1.1.356, EZH2_MOUSE, |
| EZH2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [10A2] |
| Molecular Weight: | 82 kDa |
| UniProt: | Q15910 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | EZH2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: HeLa, Jurkat, MCF7, Neuro-2a, 3T3, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 85 kDa, Observed MW: 90 kDa. |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151968. |
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Sample: Lane 1: Mouse Brain Lysates, Lane 2: Mouse Cerebellum Lysates, Lane 3: Human MCF-7 cell Lysates, Lane 4: Human Hela cell Lysates, Lane 5: Human Jurkat cell Lysates, Lane 6: Human Jar cell Lysates, Lane 7: Human HL-60 cell Lysates, Lane 8: Human K562 cell Lysates, Lane 9: Human U251 cell Lysates, Primary: Anti-EZH2 (orb1151968) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 82kDa, Observed band size: 82kDa. |
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Tissue:Human cervical carcinoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151968. |
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Tissue:Mouse spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151968. |
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Tissue:Mouse testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151968. |
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Tissue:rat spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151968. |
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