SNAP25 Recombinant Mouse Monoclonal Antibody, IgG1, Unconjugated
Catalog Number:
BYT-ORB1151970
- Images (6)
| Article Name: | SNAP25 Recombinant Mouse Monoclonal Antibody, IgG1, Unconjugated |
| Biozol Catalog Number: | BYT-ORB1151970 |
| Supplier Catalog Number: | orb1151970 |
| Alternative Catalog Number: | BYT-ORB1151970-25,BYT-ORB1151970-50,BYT-ORB1151970-100 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human SNAP25 |
| Conjugation: | Unconjugated |
| Alternative Names: | CMS18, DEE117, RIC-4, RIC4, SEC9, SNAP, SNAP-25, SUP, bA416N4.2, dJ1068F16.2, Bdr, GENA70, sp, SNAP-25B, SNAP-25a, SNP25_HUMAN, SNAP25, Super protein (SUP), Synaptosomal-associated 25 kDa protein, SNP25_MOUSE, SNP25_RAT, |
| SNAP25 Recombinant Mouse Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Molecular Weight: | 23 kDa |
| Isotype: | IgG1 |
| UniProt: | P60880 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SNAP25 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:20-100, IHC-F=1:400-800, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: Mouse brain, Rat brain, Protein loading quantity: 20 µg, Exposure time: 15 s, Predicted MW: 23 kDa, Observed MW: 25 kDa. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (orb1151970) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (orb1151970) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SNAP25) Monoclonal Antibody, Unconjugated (orb1151970) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Tissue: Human cerebellum, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151970. |
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Tissue: Mouse cerebrall cortex, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151970. |
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