CD79b Recombinant Rabbit Monoclonal Antibody, Clone: [C7H9], Unconjugated
Catalog Number:
BYT-ORB1151973
- Images (7)
| Article Name: | CD79b Recombinant Rabbit Monoclonal Antibody, Clone: [C7H9], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1151973 |
| Supplier Catalog Number: | orb1151973 |
| Alternative Catalog Number: | BYT-ORB1151973-25,BYT-ORB1151973-50,BYT-ORB1151973-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, WB |
| Species Reactivity: | Human |
| Immunogen: | A synthesized peptide derived from human CD79b (3-54aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | AGM6, B29, IGB, Igbeta, Ig-beta, CD79B_HUMAN, CD79B, B-cell-specific glycoprotein B29, Immunoglobulin-associated B29 protein, CD79B_MOUSE, |
| CD79b Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [C7H9] |
| Molecular Weight: | 23 kDa |
| UniProt: | P40259 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CD79B |
| Application Dilute: | WB=1:1000-5000, ICC/IF=1:100-500, Flow-Cyt=1:100-200 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: Raji, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 26 kDa, Observed MW: 35-40 kDa. |
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Paraformaldehyde-fixed, paraffin embedded (rat spleen), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CD79b) Monoclonal Antibody, Unconjugated (orb1151973) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human Raji cell Lysates, Primary: Anti-CD79b (orb1151973) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 23kDa, Observed band size: 35kDa. |
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Tissue: Human cervical carcinoma, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151973. |
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Tissue: Human spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151973. |
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Tissue: Mouse spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151973. |
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Tissue: Rat spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151973. |
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