XRCC5/Ku80 Recombinant Rabbit Monoclonal Antibody, Clone: [K8C2], Unconjugated
Catalog Number:
BYT-ORB1151974
- Images (7)
| Article Name: | XRCC5/Ku80 Recombinant Rabbit Monoclonal Antibody, Clone: [K8C2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1151974 |
| Supplier Catalog Number: | orb1151974 |
| Alternative Catalog Number: | BYT-ORB1151974-25,BYT-ORB1151974-50,BYT-ORB1151974-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human XRCC5/Ku80 |
| Conjugation: | Unconjugated |
| Alternative Names: | KARP-1, KARP1, KU80, KUB2, Ku86, NFIV, CTC85, CTCBF, Kup80, XRCC5_HUMAN, XRCC5, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit (CTC85 | CTCBF), DNA repair protein XRCC5, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen (TLAA), X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining), 3.6.4.-, G22P2, XRCC5_MOUSE, Ku autoantigen protein p86 homolog, |
| XRCC5/Ku80 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [K8C2] |
| Molecular Weight: | 83 kDa |
| UniProt: | P13010 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | XRCC5 |
| Application Dilute: | IHC-P=1:100-1000, IHC-F=1:100-1000, ICC/IF=1:100-500, IF=1:100-1000 |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151974. |
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Cell line: NIH/3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1151974. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (XRCC5/Ku80) monoclonal Antibody, Unconjugated (orb1151974) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (XRCC5/Ku80) Monoclonal Antibody, Unconjugated (orb1151974) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (XRCC5/Ku80) Monoclonal Antibody, Unconjugated (orb1151974) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Tissue:Mouse colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151974. |
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Tissue:Mouse stomach, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151974. |
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