MSH6 Recombinant Rabbit Monoclonal Antibody, Clone: [M5F6], Unconjugated

Catalog Number: BYT-ORB1151975
Article Name: MSH6 Recombinant Rabbit Monoclonal Antibody, Clone: [M5F6], Unconjugated
Biozol Catalog Number: BYT-ORB1151975
Supplier Catalog Number: orb1151975
Alternative Catalog Number: BYT-ORB1151975-25,BYT-ORB1151975-50,BYT-ORB1151975-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: A synthesized peptide derived from human MSH6 (1-57/1360aa)
Conjugation: Unconjugated
Alternative Names: GTBP, GTMBP, HNPCC5, HSAP, LYNCH5, MMRCS3, MSH-6, p160, MSH6_HUMAN, MSH6, hMSH6, G/T mismatch-binding protein (GTBP | GTMBP), MutS protein homolog 6, MutS-alpha 160 kDa subunit (p160), MSH6_MOUSE,
MSH6 Recombinant Rabbit Monoclonal Antibody
Clonality: Recombinant
Concentration: 1mg/ml
Clone Designation: [M5F6]
Molecular Weight: 153 kDa
UniProt: P52701
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: MSH6
Application Dilute: WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800, Flow-Cyt=1ug/Test
Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:100, Primary incubation condition: 1 hour at room temperature, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1151975.
Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human 293T cell lysates, Lane 3: Human SH-SY5Y cell lysates, Primary: Anti-MSH6 (orb1151975) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 153 kDa, Observed band size: 180 kDa.
Tissue: Human colon cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
Tissue: Human colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
Tissue: Mouse liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.
Tissue: Rat testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Counter stain: Hematoxylin, Comment: Color brown is the positive signal for orb1151975.