MUC2 Recombinant Rabbit Monoclonal Antibody, Clone: [7F9], Unconjugated
Catalog Number:
BYT-ORB1151989
- Images (6)
| Article Name: | MUC2 Recombinant Rabbit Monoclonal Antibody, Clone: [7F9], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1151989 |
| Supplier Catalog Number: | orb1151989 |
| Alternative Catalog Number: | BYT-ORB1151989-25,BYT-ORB1151989-50,BYT-ORB1151989-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human MUC2 |
| Conjugation: | Unconjugated |
| Alternative Names: | MLP, MUC-2, SMUC, 2010015E03Rik, MCM, wnn, AABR07006030.1, HH-Muc, MUC2_HUMAN, MUC2, Intestinal mucin-2, MUC2_MOUSE, Colonic mucin (MCM), Secreted gel-forming mucin, MUC2_RAT, |
| MUC2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [7F9] |
| Molecular Weight: | 570 kDa |
| UniProt: | Q02817 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | MUC2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Rat colon, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 540 kDa, Observed MW: 140-170, > 250 kDa. |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 90% methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1151989. |
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Cell line: NIH/3T3, Fixative: 4% Paraformaldehyde, Permeabilization: 90% methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1151989. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MUC2) Monoclonal Antibody, Unconjugated (orb1151989) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MUC2) Monoclonal Antibody, Unconjugated (orb1151989) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human duodenum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MUC2) Monoclonal Antibody, Unconjugated (orb1151989) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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