ALDH1A1 Recombinant Rabbit Monoclonal Antibody, Clone: [AH12], Unconjugated
Catalog Number:
BYT-ORB1152015
- Images (7)
| Article Name: | ALDH1A1 Recombinant Rabbit Monoclonal Antibody, Clone: [AH12], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1152015 |
| Supplier Catalog Number: | orb1152015 |
| Alternative Catalog Number: | BYT-ORB1152015-25,BYT-ORB1152015-50,BYT-ORB1152015-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from human ALDH1A1 |
| Conjugation: | Unconjugated |
| Alternative Names: | ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, ALHDII, Ahd-2, Ahd2, Aldh1a2, E1, Aldh2, AL1A1_HUMAN, ALDH1A1, 3-deoxyglucosone dehydrogenase, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, Retinal dehydrogenase 1 (RALDH 1 | RalDH1), 1.2.1.19, 1.2.1.28, 1.2.1.3, 1.2.1.36, AL1A1_MOUSE, AL1A1_RAT, Aldh, AL1A1_SHEEP, |
| ALDH1A1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [AH12] |
| Molecular Weight: | 55 kDa |
| UniProt: | P00352 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | ALDH1A1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Lysate: HepG2, Raji, Jurkat, K562, A549, Mouse liver, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 55 kDa, Observed MW: 55 kDa. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (orb1152015) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (orb1152015) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (orb1152015) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (orb1152015) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in EDTA buffer (Ph9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (ALDH1A1) Monoclonal Antibody, Unconjugated (orb1152015) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Mouse Lung tissue lysates, Lane 4: Mouse Kidney tissue lysates, Primary: Anti-ALDH1A1 (orb1152015) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55 kDa, Observed band size: 46 kDa. |
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