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NF-L Mouse Monoclonal Antibody, IgG1, Clone: [5H1], Unconjugated Preis auf Anfrage

NF-L Mouse Monoclonal Antibody, IgG1, Clone: [5H1], Unconjugated Preis auf Anfrage

Catalog Number: BYT-ORB1152079

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Article Name:	NF-L Mouse Monoclonal Antibody, IgG1, Clone: [5H1], Unconjugated Preis auf Anfrage
Biozol Catalog Number:	BYT-ORB1152079
Supplier Catalog Number:	orb1152079
Alternative Catalog Number:	BYT-ORB1152079-1
Manufacturer:	Biorbyt
Host:	Mouse
Category:	Antikörper
Application:	ELISA
Species Reactivity:	Mouse, Rat
Immunogen:	Recombinant Human NF-L Protein (1-543/543)
Conjugation:	Unconjugated
Alternative Names:	Neurofilament L, Neurofilament 68, Neurofilament triplet L, 70 kD Neurofilament Light, 68kDa neurofilament protein, CMT 1F, CMT 2E, CMT1F, CMT2E, FLJ53642, Light molecular weight neurofilament protein, NEFL, Neurofilament light, Neurofilament light polypeptide 68kDa, Neurofilament light polypeptide, Neurofilament protein, light chain, Neurofilament subunit NF L, Neurofilament triplet L protein, NF 68, NF L, NF68, NFL, NFL_HUMAN.

NF-L Mouse Monoclonal Antibody

Clonality:	Monoclonal
Clone Designation:	[5H1]
Molecular Weight:	68 kDa
Isotype:	IgG1
UniProt:	P07196
Buffer:	0.01M PBS (pH7.4).
Form:	Liquid
Target:	NEFL

Application Dilute:

ELISA=1:5000-10000

	Measured by its binding ability in a indirect ELISA. Immobilized Human NF-L protein, His Tag at 2 µg/mL (100 µl/well) can bind Mouse Anti-Human NF-L Antibody, the EC50 is 87.36 ng/ml.
	Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (NF-L) Monoclonal Antibody, Unconjugated (orb1152079) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
	Sample: Lane 1: Recombinant Human NF-L Protein at 100 ng, Primary: Mouse Anti-NF-L Monoclonal Antibody at 1/1000 dilution (Cat.orb1152079), Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 61.4kD, Observed band size: 68/44kD.

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