GFAP Recombinant Rabbit Monoclonal Antibody, Clone: [3F4], Unconjugated
Catalog Number:
BYT-ORB1152219
- Images (7)
| Article Name: | GFAP Recombinant Rabbit Monoclonal Antibody, Clone: [3F4], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1152219 |
| Supplier Catalog Number: | orb1152219 |
| Alternative Catalog Number: | BYT-ORB1152219-25,BYT-ORB1152219-50,BYT-ORB1152219-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human GFAP (1-41aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ALXDRD, GFAP_BOVIN, GFAP, GFAP_HUMAN, GFAP_MOUSE, |
| GFAP Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [3F4] |
| Molecular Weight: | 48 kDa |
| UniProt: | P14136 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GFAP |
| Application Dilute: | WB=1:1000-50000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
|
|
Cell line: Mouse primary astrocyte, Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1152219. |
|
|
Cell line: Neuro-2a (Negative cell line), Fixation: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: No staining on orb1152219. |
|
|
Paraformaldehyde-fixed, paraffin embedded (MOUSE brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (orb1152219) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Lane 1: Rat Cerebrum tissue lysates, Lane 2: Rat Cerebellum tissue lysates, Lane 3: Human U251 cell lysates, Primary: Anti-GFAP (orb1152219) at 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 48 kDa. |
Product Guarantee and Expert Support
