Ki67/MKI67 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1152331
Article Name: Ki67/MKI67 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1152331
Supplier Catalog Number: orb1152331
Alternative Catalog Number: BYT-ORB1152331-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: ELISA, IHC
Species Reactivity: Human
Immunogen: E.coli-derived human Ki67/MKI67 recombinant protein (Position: R5-Q2358).
Conjugation: Unconjugated
Alternative Names: D630048A14Rik, Ki 67, Ki67, Mki67
Anti-Ki67/MKI67 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human.
Clonality: Polyclonal
Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Molecular Weight: 358 kDa
UniProt: P46013
Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Form: Lyophilized
Target: Proliferation marker protein Ki-67
Application Dilute: Immunohistochemistry(Paraffin-embedded Section), 2-5 µg/ml, Human ELISA, 0.1-0.5 µg/ml, -
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ki67/MKI67 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ki67/MKI67 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ki67/MKI67 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ki67/MKI67 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody. Ki67/MKI67 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ki67/MKI67 Antibody overnight at 4C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of Ki67/MKI67 using anti-Ki67/MKI67 antibody. Ki67/MKI67 was detected in a paraffin-emb