WDR26 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB11572
- Images (6)
| Article Name: | WDR26 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB11572 |
| Supplier Catalog Number: | orb11572 |
| Alternative Catalog Number: | BYT-ORB11572-50,BYT-ORB11572-100,BYT-ORB11572-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human WDR26 (101-200/514aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CDW2, GID7, MIP2, SKDEAS, 1600024A01Rik, RGD1565589, WDR26_HUMAN, WDR26, CUL4- and DDB1-associated WDR protein 2, Myocardial ischemic preconditioning up-regulated protein 2, WDR26_MOUSE, |
| WDR26 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 72 kDa |
| UniProt: | Q9H7D7 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | WDR26 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green). |
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Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-WDR26 antibody (orb11572), Dilution: 1 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-WDR26 (orb11572) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 72 kD, Observed band size: 70 kD. |
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Tissue/Cell: human laryngocarcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated (orb11572) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: MCF7, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (WDR26) Polyclonal Antibody, Unconjugated (orb11572) 1:200, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Tissue/Cell: rat heart tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-WDR26 Polyclonal Antibody, Unconjugated (orb11572) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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