SF1 Recombinant Rabbit Monoclonal Antibody, Clone: [E1F12], Unconjugated
Catalog Number:
BYT-ORB1172521
- Images (7)
| Article Name: | SF1 Recombinant Rabbit Monoclonal Antibody, Clone: [E1F12], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1172521 |
| Supplier Catalog Number: | orb1172521 |
| Alternative Catalog Number: | BYT-ORB1172521-25,BYT-ORB1172521-50,BYT-ORB1172521-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | BBP, D11S636, MBBP, ZCCHC25, ZFM1, ZNF162, MZFM, WBP4, Zfp162, SF01_HUMAN, SF1, Mammalian branch point-binding protein (BBP | mBBP), Transcription factor ZFM1, Zinc finger gene in MEN1 locus, Zinc finger protein 162, SF01_MOUSE, CW17, Transcription factor ZFM1 (mZFM), |
| SF1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [E1F12] |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SF1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: HepG2, 3: PC-12, 4: NIH-3T3, 5: Mouse testis, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 52 kDa, Observed MW: 50-75 kDa. |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172521. |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172521. |
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Cell line: Neuro-2a, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1172521. |
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Tissue: Human testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172521. |
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Tissue: Mouse testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172521. |
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Tissue: Rat testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172521. |
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