Myosin light chain 3 Recombinant Rabbit Monoclonal Antibody, Clone: [F12A11], Unconjugated
Catalog Number:
BYT-ORB1172794
- Images (7)
| Article Name: | Myosin light chain 3 Recombinant Rabbit Monoclonal Antibody, Clone: [F12A11], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1172794 |
| Supplier Catalog Number: | orb1172794 |
| Alternative Catalog Number: | BYT-ORB1172794-25,BYT-ORB1172794-50,BYT-ORB1172794-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | CMH8, MLC-lV/sb, MLC1SB, MLC1V, VLC1, VLCl, MLC1s, Mylc, MLClV, Mylc1v, rVMLC1, MYL3_HUMAN, MYL3, Cardiac myosin light chain 1 (CMLC1), Myosin light chain 1, slow-twitch muscle B/ventricular isoform (MLC1SB), Ventricular myosin alkali light chain, Ventricular myosin light chain 1 (VLCl), Ventricular/slow twitch myosin alkali light chain (MLC-lV/sb), MYL3_MOUSE, Myosin alkali light chain 1, ventricular/slow skeletal muscle isoform, MYL3_RAT, Myosin alkali light chain 1, ventricular (MLClV), Ventricular myosin light chain 1 (rVMLC1), |
| Myosin light chain 3 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [F12A11] |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | MYL3 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Mouse heart, Rat heart, Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 22 kDa, Observed MW: 22 kDa. |
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Cell line: U-2 OS, Fixative: 4% Paraformaldehyde, Permeabilization: 90% methanol, Primary ab Dilution: 1:100, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172794. |
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Tissue: Human skeletal muscle, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172794. |
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Tissue: Mouse cardiac muscle, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172794. |
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Tissue: Mouse skeletal muscle, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172794. |
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Tissue: Rat cardiac muscle, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172794. |
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Tissue: Rat skeletal muscle, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:1000, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172794. |
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