EEF1A1 Recombinant Mouse Monoclonal Antibody, Clone: [C2C12], Unconjugated
Catalog Number:
BYT-ORB1172823
- Images (6)
| Article Name: | EEF1A1 Recombinant Mouse Monoclonal Antibody, Clone: [C2C12], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1172823 |
| Supplier Catalog Number: | orb1172823 |
| Alternative Catalog Number: | BYT-ORB1172823-25,BYT-ORB1172823-50,BYT-ORB1172823-100 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, EF1A1, EF1alpha1, GRAF-1EF, LENG7, PTI1, eEF1A-1, Eef1a2, Eef1a2l1, SI, EF1A1_HUMAN, EEF1A1, EF-1-alpha-1, Elongation factor Tu (EF-Tu), Eukaryotic elongation factor 1 A-1 (eEF1A-1), Leukocyte receptor cluster member 7, 3.6.5.-, EF1A1_MOUSE, EF1A1_RAT, |
| EEF1A1 Recombinant Mouse Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [C2C12] |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | EEF1A1 |
| Application Dilute: | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: MCF-7, Daudi, 4T1, BRL, Protein loading quantity: 20 µg, Exposure time: 1 s, Predicted MW: 50 kDa, Observed MW: 50 kDa. |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: MCF-7, Daudi, 4T1, BRL, Protein loading quantity: 20 µg, Exposure time: 1 s, Predicted MW: 50 kDa, Observed MW: 50 kDa. |
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Cell line: MCF7, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172823. |
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Tissue: Human breast cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172823. |
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Tissue: Mouse brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172823. |
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Tissue: Rat liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172823. |
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