CD62L Recombinant Rabbit Monoclonal Antibody, Clone: [G3B6], Unconjugated
Catalog Number:
BYT-ORB1172983
- Images (6)
| Article Name: | CD62L Recombinant Rabbit Monoclonal Antibody, Clone: [G3B6], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1172983 |
| Supplier Catalog Number: | orb1172983 |
| Alternative Catalog Number: | BYT-ORB1172983-25,BYT-ORB1172983-50,BYT-ORB1172983-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, WB |
| Species Reactivity: | Human |
| Conjugation: | Unconjugated |
| Alternative Names: | CD62L, L-selectin, LAM-1, LECAM-1, LECAM1, Lnhr, Ly-22, Ly-m22, Lyam-1, Lyam1, LYAM1_MOUSE, Sell, CD62 antigen-like family member L, Leukocyte adhesion molecule 1 (LAM-1), Leukocyte-endothelial cell adhesion molecule 1 (LECAM1), Lymph node homing receptor, Lymphocyte antigen 22 (Ly-22), Lymphocyte surface MEL-14 antigen, Ly22, LAM1, LEU8, LSEL, PLNHR, TQ1, LYAM1_HUMAN, Leukocyte surface antigen Leu-8, gp90-MEL, |
| CD62L Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [G3B6] |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | SELL |
| Application Dilute: | WB=1:500-1000, ICC/IF=1:100-500, Flow-Cyt=1:50 |
|
|
Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Human serum, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 42 kDa, Observed MW: 100 kDa. |
|
|
Cell line: EL4, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983. |
|
|
Cell line: Jurkat, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983. |
|
|
Cell line: PBMC, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Secondary ab: Goat anti Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1172983. |
|
|
Cell line: Raji, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983. |
|
|
Cell line: Ramos, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172983. |
Product Guarantee and Expert Support
