BAG6 Recombinant Mouse Monoclonal Antibody, Clone: [B5E6], Unconjugated
Catalog Number:
BYT-ORB1173019
- Images (6)
| Article Name: | BAG6 Recombinant Mouse Monoclonal Antibody, Clone: [B5E6], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1173019 |
| Supplier Catalog Number: | orb1173019 |
| Alternative Catalog Number: | BYT-ORB1173019-25,BYT-ORB1173019-50,BYT-ORB1173019-100 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | BAG6_HUMAN, BAG6, BAG family molecular chaperone regulator 6, BCL2-associated athanogene 6 (BAG-6), HLA-B-associated transcript 3, Protein G3, Protein Scythe, BAT3, G3, BAG6_ARATH, Bcl-2-associated athanogene 6, CaM-binding protein 1, CAMBP1, BAG6_RAT, BCL2-associated athanogene 6, BAG-6, D6S52E, 2410045D21Rik, D17H6S52E, Scythe, Bat-3, bag6-a, bag6-b, wu:fi30e10, |
| BAG6 Recombinant Mouse Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [B5E6] |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BAG6 |
| Application Dilute: | WB=1:500-1000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: HeLa, 2: SH-SY5Y, Protein loading quantity: 20 µg, Exposure time: 3 s, Predicted MW: 150 kDa, Observed MW: 150 kDa. |
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: RAW 267.7, 2: A20, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 150 kDa, Observed MW: 150 kDa. |
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Cell line: HeLa, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1173019. |
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Tissue: Human breast cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173019. |
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Tissue: Mouse testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173019. |
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Tissue: Rat kidney, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173019. |
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