ANKRD33B Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB1212
Article Name: ANKRD33B Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB1212
Supplier Catalog Number: orb1212
Alternative Catalog Number: BYT-ORB1212-50,BYT-ORB1212-100,BYT-ORB1212-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human ANKRD33B (401-494/494aa)
Conjugation: Unconjugated
Alternative Names: DP58, AN34B_HUMAN, ANKRD34B,
ANKRD33B Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 54 kDa
UniProt: A5PLL1
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: ANKRD34B
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated (orb1212) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated (orb1212) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ANKRD33B) Polyclonal Antibody, Unconjugated (orb1212) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Cerebellum (Mouse) Lysate at 40 ug, Cerebellum (Rat) Lysate at 40 ug, Hippocampus (Mouse) Lysate at 40 ug, Primary: Anti-ANKRD33B (orb1212) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.



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