Application Notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 44.2 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human 4-1BB Ligand, Fc Tag at 1 ug/ml (100 uL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.6-5 ng/mL.
Immobilized Human 4-1BB Ligand, His Tag at 2 ug/ml (100 uL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.1-4 ng/mL.
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