Application Notes: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 27.5 kDa. The protein migrates as 43-55 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation
Biotinylated Human Syndecan-1, His, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human Syndecan-1, His, Avitag at 1 ug/ml (100 uL/well) on streptavidin (0.5 ug/well) plate, can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.4-6 ng/mL.
Immobilized Anti-Syndecan-1 antibody, Mouse IgG1 at 1 ug/ml (100 uL/well) onstreptavidin (0.1 ug/well) plate, can bind Biotinylated Human Syndecan-1, His, Avitag with a linear range of 2-63 ng/mL.
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