Application Notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.7 kDa. The protein migrates as 22-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation
Human PVRIG, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human Nectin-2, Fc Tag at 5 ug/ml (100 uL/well) can bind Human PVRIG Protein, His Tag with a linear range of 0.02-0.156 ug/ml.
Immobilized Biotinylated Human Nectin-2, Fc, Avitag at 1 ug/ml (100 uL/well) on streptavidin precoated (0.5 ug/well) plate, can bind Human PVRIG Protein, His Tag with a linear range of 10-78 ng/mL.
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