IL-17RA antibody was raised against an 18 amino acid peptide near the amino terminus of human IL-17RA.The immunogen is located within amino acids 150 - 200 of IL-17RA.
Conjugation:
Unconjugated
Alternative Names:
IL-17RA Antibody: CD217, IL17R, CANDF5, CDw217, IL-17RA, hIL-17R, Interleukin-17 receptor A, IL-17 receptor A
IL-17RA Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
IL17RA
Application Notes:
Application Notes: IL-17RA antibody can be used for detection of IL-17RA by Western blot at 1 - 2 µg/mL.Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested
Western blot analysis of IL17RA in A20 cell lysate with IL17RA antibody at 1 µg/mL.
WB Validation in K562 Cells. Loading: 10 µg of lysate. Antibodies: IL-17RA, orb1238913, 4 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry of IL-17RA in human spleen tissue with IL-17RA antibody at 2.5 µg/ml.
WB Validation in Mouse Tissues. Loading: 15 µg of lysate. Antibodies: IL-17RA, orb1238913, 2 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Immunofluorescence Validation of IL-17RA in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen tissue labeling IL-17RA with orb1238913 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
Immunofluorescence Validation of IL-17RA in Mouse Thymus. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus tissue labeling IL-17RA with orb1238913 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
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