B7-H3 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
Form:
Liquid
Target:
CD276
Application Notes:
Application Notes: B7-H3 antibody can be used for detection of B7-H3 for immunohistochemistry starting at 2 µg/mL and Immunocytochemistry starting at 1 µg/mL. For immunofluorescence start at 10 µg/mL. Flow cytometry at 1 µg/ml.Antibody validated: Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in human samples. All other applications and species not yet tested
Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 µg/ml.
Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 µg/ml. Green: B7-H3 Antibody [7B3] (orb1239247) Blue: DAPI staining
Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 µg/ml. Green: B7-H3 Antibody [7B3] (orb1239247) Blue: DAPI staining
Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 µg/ml.
Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 µg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.
Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA with orb1240155, orb1239243, orb1239244, orb1239247, and orb1239231 antibodies at decreasing concentrations.
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