Anti- Caspase 9 antibody (orb1239266) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9. The immunogen is located within amino acids 30-80 of Caspase 9.
Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
CASP9
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (1 µg/mL), Caspase 9, orb1239265 (1 µg/mL) and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL.
Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL.
Western Blot Validation in Rat Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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