Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
VCL
Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240276 (2 µg/mL), Vinculin orb1240285 (1 µg/mL), Vinculin orb1240274 (1 µg/mL), and beta-actin orb1240312 (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in PC3 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Liver Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Liver Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: Vinculin orb1240285 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunocytochemistry Validation of Vinculin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (orb1240285) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of Vinculin in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with orb1240285 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
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