Anti-ZIP7 antibody (orb1240303) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7. The immunogen is located within amino acids 20-70 of ZIP7.
ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Form:
Liquid
Target:
SLC39A7
Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (1 µg/mL), ZIP7 (4 µg/mL), and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Mouse Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: ZIP7, orb1240303 (Lane 1: 0.5 µg/mL and Lane 2: 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of ZIP7 in Human Brain Tissue. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (orb1240303) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT, antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunofluorescence Validation of ZIP7 in Human Brain Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with orb1240303 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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