Integrin beta 1 Rabbit Polyclonal Antibody (PE)
Catalog Number:
BYT-ORB124704
- Images (6)
| Article Name: | Integrin beta 1 Rabbit Polyclonal Antibody (PE) |
| Biozol Catalog Number: | BYT-ORB124704 |
| Supplier Catalog Number: | orb124704 |
| Alternative Catalog Number: | BYT-ORB124704-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Integrin beta 1 (25-100/798aa) |
| Conjugation: | PE |
| Alternative Names: | Integrin beta-1, beta 1 subunit, CD 29, CD-29, CD29, CD29 antigen, Fibrinogen Receptor beta subunit, Fibronectin receptor subunit beta, Fibronectin receptor, beta subunit, FNRB, GPIIA, GP IIa, Integrin VLA 4 subunit beta, ITGB, ITGB1, ITGB 1, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, Glycoprotein IIa, Integrin VLA 4 subunit beta, integrin VLA-4 beta subunit, ITB1_HUMAN, very late activation protein, beta polypeptide, VLA BETA, VLA-4 subunit beta, VLA-BETA, VLAB, VLAbeta, Integrin beta1. |
| Integrin beta 1 Rabbit Polyclonal Antibody (PE) is a PE conjugated antibody targeting ITGB1. This antibody is suitable for FC, IF. It exhibits reactivity with Human, Mouse, Rat samples. |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Target: | ITGB1 |
| Application Dilute: | Flow-Cyt=1µg/Test, IF=1:100-500 |
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Blank control: A431. Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (orb124704), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: A549. Primary Antibody (green line): Rabbit Anti-CD29 antibody (orb124704), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (orb124704), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (orb124704), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-CD29 antibody (orb124704), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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Blank control: U87MG. Primary Antibody (green line): Rabbit Anti-Integrin beta 1 antibody (orb124704), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. |
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