DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing.
Form:
Liquid
Target:
DAP3
Application Notes:
Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 40kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1µg/mL. Primary incubated 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10µg/mL
orb1247986 (0.3 ug/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.
orb1247986 (1 ug/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35 ug protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR.
orb1247986 (2.5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1247986 (2.5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1247986 staining (0.3 ug/ml) of Human Kidney lysate (RIPA buffer, 30 ug total protein per lane). Detected by chemiluminescence.
orb1247986 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).
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