PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Form:
Liquid
Target:
ENO2
Application Notes:
Application Notes: Western blot: 0.5-1 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 µg/mLIHC (FFPE): 0.1-0.2 µg/mL for 30 min at RTThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance
IHC testing of FFPE human cerebellum with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE human pheochromocytoma with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
Western blot testing of human 1) Y79, 2) HeLa and 3) HepG2 cell lysate with NSE antibody (clone ENO2/1375). Predicted molecular weight ~47 kDa.
IHC testing of FFPE mouse pancreas with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
IHC testing of FFPE rat heart with NSE antibody (clone ENO2/1375). Required HIER: boil sections in 10mM citrate buffer, pH6, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free NSE Antibody (clone ENO2/1375). Confirmation of Integrity and Purity of the Antibody.
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