PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Form:
Liquid
Target:
CDH1
Application Notes:
Application Notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLWestern blot: 0.5-1 µg/mLImmunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (1)Optimal dilution of the E-Cadherin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with E-Cadherin antibody (CDH1/1525).
Formalin-fixed, paraffin-embedded human prostate carcinoma stained with E-Cadherin antibody (CDH1/1525).
Formalin-fixed, paraffin-embedded human skin stained with E-Cadherin antibody (CDH1/1525).
Formalin-fixed, paraffin-embedded human placenta stained with E-Cadherin antibody (CDH1/1525).
Western blot analysis A) partial recombinant protein B) human stomach lysate using E-Cadherin antibody (CDH1/1525). Expected molecular weight: 135 kDa (precursor), 80-120 kDa (mature, depending on gylcosylation level).
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