PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Form:
Liquid
Target:
MFGE8
Application Notes:
Application Notes: Western blot: 1-2 µg/mLFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the MFGE8 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFGE8 antibody (MFG-06).
FFPE human pancreas tested with MFGE8 antibody (MFG-06).
FFPE human lung carcinoma tested with MFGE8 antibody (MFG-06).
FFPE human renal cell carcinoma tested with MFGE8 antibody (MFG-06).
Western blot testing of MCF7 cell lysate with MFGE8 antibody (clone MFG-06).
SDS-PAGE Analysis of Purified, BSA-Free MFGE8 Antibody (clone MFG-06). Confirmation of Integrity and Purity of the Antibody.
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