PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Form:
Liquid
Target:
PECAM1
Application Notes:
Application Notes: Flow Cytometry: 0.5-1ug/10e6 cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min
IHC staining of colon carcinoma with PECAM-1 antibody (C31.7). Note cell membrane staining of endothelial cells.
IHC staining of angiosarcoma with PECAM-1 antibody (C31.7).
IHC staining of tonsil tissue with PECAM-1 antibody (C31.7).
Western blot testing of human samples using PECAM-1 antibody (C31.7).
FACS testing of Jurkat cells: Black = cells alone, Green = isotype control, Red = PECAM-1 antibody PE conjugate.
SDS-PAGE Analysis of Purified, BSA-Free PECAM-1 Antibody (clone C31.7). Confirmation of Integrity and Purity of the Antibody.
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