ACAT1 Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1255472
- Images (7)
| Article Name: | ACAT1 Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1255472 |
| Supplier Catalog Number: | orb1255472 |
| Alternative Catalog Number: | BYT-ORB1255472-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1). |
| Conjugation: | Unconjugated |
| Alternative Names: | acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1 |
| ACAT1 Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 42kDa |
| UniProt: | P24752 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | ACAT1 |
| Application Notes: | Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
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Western blot analysis of extracts of various cell lines, using ACAT1 antibody (orb1255472) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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Immunohistochemistry of paraffin-embedded human lung cancer using ACAT1 antibody (orb1255472) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded rat spleen using ACAT1 antibody (orb1255472) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse lung using ACAT1 antibody (orb1255472) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of C6 cells using ACAT1 antibody (orb1255472) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (orb1255472) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (orb1255472) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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