DDX50 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1255736
Article Name: DDX50 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1255736
Supplier Catalog Number: orb1255736
Alternative Catalog Number: BYT-ORB1255736-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human DDX50 (NP_076950.1).
Conjugation: Unconjugated
Alternative Names: DDX50, GU2, GUB, mcdrh, RH-II/GuB
DDX50 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 105kDa
UniProt: Q9BQ39
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DDX50
Application Notes: Application Notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DDX50 antibody (orb1255736) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse testis using DDX50 antibody (orb1255736) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using DDX50 antibody (orb1255736) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using DDX50 antibody (orb1255736) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using DDX50 antibody (orb1255736) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using DDX50 antibody (orb1255736) at dilution of 1:100. Blue: DAPI for nuclear staining.