MDC1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1255888
Article Name: MDC1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1255888
Supplier Catalog Number: orb1255888
Alternative Catalog Number: BYT-ORB1255888-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MDC1 (NP_055456.2).
Conjugation: Unconjugated
Alternative Names: IER3, NFBD1, KIAA0170, DKFZp781A0122
MDC1 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 270kDa
UniProt: Q14676
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: MDC1
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:100 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using MDC1 antibody (orb1255888) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using MDC1 antibody (orb1255888) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MDC1 antibody (orb1255888) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using MDC1 antibody (orb1255888) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using MDC1 antibody (orb1255888) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MDC1 antibody (orb1255888) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using MDC1 antibody (orb1255888) at dilution of 1:100. Blue: DAPI for nuclear staining.