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RIPK1 Antibody, Unconjugated, Rabbit, Polyclonal

RIPK1 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256411

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Article Name:	RIPK1 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1256411
Supplier Catalog Number:	orb1256411
Alternative Catalog Number:	BYT-ORB1256411-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-440 of human RIPK1 (NP_003795.2).
Conjugation:	Unconjugated
Alternative Names:	RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1

RIPK1 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 76kDa
UniProt:	Q13546
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	RIPK1

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using RIPK1 antibody (orb1256411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
	Western blot analysis of extracts of various cell lines, using RIPK1 antibody (orb1256411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded human tonsil using RIPK1 antibody (orb1256411) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat lung using RIPK1 antibody (orb1256411) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1 antibody (orb1256411) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using RIPK1 antibody (orb1256411) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using RIPK1 antibody (orb1256411) at dilution of 1:100. Blue: DAPI for nuclear staining.

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