H4K8ac Antibody, Unconjugated, Rabbit, Polyclonal
Catalog Number:
BYT-ORB1256500
- Images (7)
| Article Name: | H4K8ac Antibody, Unconjugated, Rabbit, Polyclonal |
| Biozol Catalog Number: | BYT-ORB1256500 |
| Supplier Catalog Number: | orb1256500 |
| Alternative Catalog Number: | BYT-ORB1256500-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ChIP, IF, IHC, IP, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | A synthetic peptide of human Acetyl-Histone H4-K8 |
| Conjugation: | Unconjugated |
| Alternative Names: | H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, H4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4, Histone H4H4FA |
| H4K8ac Antibody |
| Clonality: | Polyclonal |
| Concentration: | batch dependent |
| Molecular Weight: | Observed: 11kDa |
| UniProt: | P62805 |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Form: | Liquid |
| Target: | H4K8ac |
| Application Notes: | Application Notes: WB: 1:1000 - 1:3000IHC: 1:200 - 1:500IF: 1:500 - 1:1000IP: 1:200 - 1:500CHIP: 1:20 - 1:50CHIP seq: 1:20 - 1:50 |
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Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K8 antibody (orb1256500) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s. |
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Immunohistochemistry of paraffin-embedded rat ovary using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human breast using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded human gastric cancer using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens). |
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Immunohistochemistry of paraffin-embedded mouse brain using H4K8ac antibody (orb1256500) at dilution of 1:100 (40x lens). |
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Immunofluorescence analysis of HeLa cells using Acetyl-Histone H4-K8 antibody (orb1256500). Blue: DAPI for nuclear staining. |
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Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H4K8ac antibody (orb1256500) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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