Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (orb1256506) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K27 antibody (orb1256506) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (orb1256506) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
* VAT and and shipping costs not included. Errors and price changes excepted
