MAP2K5 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256700
Article Name: MAP2K5 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256700
Supplier Catalog Number: orb1256700
Alternative Catalog Number: BYT-ORB1256700-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human MAP2K5 (NP_660143.1).
Conjugation: Unconjugated
Alternative Names: Dual specificity mitogen-activated protein kinase kinase 5, MAP kinase kinase 5, MAPKK 5, MAPK/ERK kinase 5, MEK 5, MAP2K5, MEK5, MKK5, PRKMK5
MAP2K5 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 50kDa
UniProt: Q13163
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: MAP2K5
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using MAP2K5 antibody (orb1256700) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody (orb1256700) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody (orb1256700) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody (orb1256700) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody (orb1256700) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MAP2K5 antibody (orb1256700). Blue: DAPI for nuclear staining.