DDX41 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256921
Article Name: DDX41 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256921
Supplier Catalog Number: orb1256921
Alternative Catalog Number: BYT-ORB1256921-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human DDX41 (NP_057306.2).
Conjugation: Unconjugated
Alternative Names: DDX41 Antibody: ABS, ABS, Probable ATP-dependent RNA helicase DDX41, DEAD box protein 41
DDX41 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 75kDa
UniProt: Q9UJV9
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: DDX41
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using DDX41 antibody (orb1256921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using DDX41 antibody (orb1256921) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using DDX41 antibody (orb1256921) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DDX41 antibody (orb1256921) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using DDX41 antibody (orb1256921). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug DDX41 antibody (orb1256921). Western blot was performed from the immunoprecipitate using DDX41 antibody (orb1256921) at a dilution of 1:1000.