CIRBP Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256933
Article Name: CIRBP Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256933
Supplier Catalog Number: orb1256933
Alternative Catalog Number: BYT-ORB1256933-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CIRBP (NP_001271.1).
Conjugation: Unconjugated
Alternative Names: CIRBP, CIRP
CIRBP Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 18kDa
UniProt: Q14011
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: CIRBP
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:10 - 1:100
Western blot analysis of extracts of various cell lines, using CIRBP antibody (orb1256933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CIRBP antibody (orb1256933) at dilution of 1:100 (20x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CIRBP antibody (orb1256933) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CIRBP antibody (orb1256933). Blue: DAPI for nuclear staining.