CCT4 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1256940
Article Name: CCT4 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1256940
Supplier Catalog Number: orb1256940
Alternative Catalog Number: BYT-ORB1256940-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-390 of human CCT4 (NP_006421.2).
Conjugation: Unconjugated
Alternative Names: CCT4, SRB, Cctd, CCT-DELTA
CCT4 Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 60kDa
UniProt: P50991
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: CCT4
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
Western blot analysis of extracts of various cell lines, using CCT4 antibody (orb1256940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat stomach using CCT4 antibody (orb1256940) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CCT4 antibody (orb1256940) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using CCT4 antibody (orb1256940) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CCT4 antibody (orb1256940) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CCT4 antibody (orb1256940). Blue: DAPI for nuclear staining.