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MRPL28 Antibody, Unconjugated, Rabbit, Polyclonal

MRPL28 Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257261

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Article Name:	MRPL28 Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:	BYT-ORB1257261
Supplier Catalog Number:	orb1257261
Alternative Catalog Number:	BYT-ORB1257261-100
Manufacturer:	Biorbyt
Host:	Rabbit
Category:	Antikörper
Application:	IF, IHC, IP, WB
Species Reactivity:	Human, Rat
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-256 of human MRPL28 (NP_006419.2).
Conjugation:	Unconjugated
Alternative Names:	39S ribosomal protein L28, mitochondrial, L28mt, MRP-L28, Melanoma antigen p15, Melanoma-associated antigen recognized by T-lymphocytes, MRPL28, MAAT1

MRPL28 Antibody

Clonality:	Polyclonal
Concentration:	batch dependent
Molecular Weight:	Observed: 30kDa
UniProt:	Q13084
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form:	Liquid
Target:	MRPL28

Application Notes:

Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200

	Western blot analysis of extracts of various cell lines, using MRPL28 antibody (orb1257261) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
	Immunohistochemistry of paraffin-embedded rat ovary using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using MRPL28 antibody (orb1257261) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using MRPL28 antibody (orb1257261). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200 ug extracts of 293T cells using 1 ug MRPL28 antibody (orb1257261). Western blot was performed from the immunoprecipitate using MRPL28 antibody (orb1257261) at a dilution of 1:1000.

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