Western blot analysis of extracts of various cell lines, using RPS3A antibody (orb1257271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (orb1257271). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 3 ug RPS3A antibody (orb1257271). Western blot was performed from the immunoprecipitate using RPS3A antibody (orb1257271) at a dilution of 1:500.
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