RPS3A Antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: BYT-ORB1257271
Article Name: RPS3A Antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: BYT-ORB1257271
Supplier Catalog Number: orb1257271
Alternative Catalog Number: BYT-ORB1257271-100
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
Conjugation: Unconjugated
Alternative Names: 40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255|HAMAP-Rule:MF_03122}, FTE1, MFTL
RPS3A Antibody
Clonality: Polyclonal
Concentration: batch dependent
Molecular Weight: Observed: 35kDa
UniProt: P61247
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Form: Liquid
Target: RPS3A
Application Notes: Application Notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using RPS3A antibody (orb1257271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (orb1257271). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 3 ug RPS3A antibody (orb1257271). Western blot was performed from the immunoprecipitate using RPS3A antibody (orb1257271) at a dilution of 1:500.